Vankomisin kaynaklı nefrotoksisiteyi önlemede melatoninin etkinliği: deneysel bir çalışma

Purpose: The aim of the study explores probable toxic effects vancomycin on kidney and analysis protective melatonin.
 Materials Methods: In this study, rats were randomly divided into 4 groups: control group; melatonin (10 mg/kg/day) vancomycin-treated (200 mg/kg) + group. Rats in treatment group given two doses a day with an interval seven consecutive days once daily for days. experiment was continued 15 each group, grouped together. after experiment, sacrificed under anesthesia among all groups. Kidney tissues collected processed further TNF- expression analysis, as well histological analyses such hematoxylin eosin (H&E), Masson's tricrom, Periodic acid schiff (PAS) staining to assess pathological severity. addition, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay performed evaluate apoptosis.
 Results: While upregulated TNF-α expression, reduced levels immunoreactivity intensity clearly improved severity rat kidneys. Further, significantly inhibited vancomycin-induced TUNEL-positive cell numbers. 
 Conclusion: Melatonin has activity against pro-inflammatory proapoptotic kidneys during organ preservation time improves function.